GrainGenes Marker Report: Xwmc498-2B
Locus
Xwmc498-2B
Type
Microsatellite
Chromosome
2B
Map
Ta-SSR-2004-2B 64.1
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC498
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
wmc498 wmc498-2B
Probe for Locus
WMC498
Locus
Xwmc498-2B wmc498 wmc498-2B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 25e11 Donor: Serasem Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 136 bases located on 2B Trimmed to insert at both ends. Bases 1 to 32 show homology to 2b1 bases 76 to 107 (32 in longest run, maxScore = 36). (GT)14 50 to 77, (GT)6 80 to 91, (GT)6 94 to 105, (GC)5 106 to 115.
Type
PCR SSR
PCR primers
Forward cgATgAAgAgAgccATcAAAA at 27 length 21 bases, Tm 62
Reverse TgAcATTccggTAggTcAgTT at 142 length 21 bases, Tm 61
SSR size
136 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide (134 bases - gives null alleles).
Sequence
WMC498
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc498-2B
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