GrainGenes Marker Report: Xwmc50-3A
Locus
Xwmc50-3A
Type
Microsatellite
Map
Conan/Reeder-3A 34.1164 Kofa/UC1113-3A 28.2
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Conan x Reeder
Probe
WMC50
Possible Orthologs
Xwmc050 Xwmc50 wmc50 wmc50-3A
Probe for Locus
WMC50
Locus
Xwmc50-3A Xwmc50 Xwmc050 wmc50 wmc50-3A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6e5 Donor: Dubcovsky, Buenos Aires AGR85. Trimmed to insert at 3' end. Multiple inserts (2) joined 219-236 by linkers. Microsatelilite in first insert too close to end to make primers. First insert matches 7B1. Second insert (GT)10 and (GT)16 repeats 402 - 456. Primers are to second insert.
Type
PCR SSR
PCR primers
Forward cTgccgTcAggccAggcTcAcA at 349 length 22 bases, Tm 76
Reverse cAAccAgcTAgcTgccgccgAA at 472 length 22 bases, Tm 74
Amplification Conditions
61C anneal, product;
Sequence
WMC50
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc50-3A
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