GrainGenes Marker Report: Xwmc501.2
Locus
Xwmc501.2
Type
Microsatellite
Map
Ta-NxW-4A 104.3
[ Show Nearby Loci ]
Map Data
Wheat, Nanda2419 x Wangshuibai
Probe
WMC501
Possible Orthologs
Xwmc501.1
Probe for Locus
WMC501
Locus
Xwmc501.2 Xwmc501.1
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 20b7 Donor: Byrne, Colorado State Trimmed to insert at 3' end, 5' end not located. Bases 241 to 295 show homology to 4f3 bases 112 to 166 (55 in longest run, maxScore = 61), forward primer is not within this region of homology, reverse primer matches 4f3. (GT)30 244 to 303.
Type
PCR SSR
PCR primers
Forward cTcggcTTcAcTATcgTcgTcT at 105 length 22 bases, Tm 65
Reverse TAcTTgTcAcATcggcccTcTT at 306 length 22 bases, Tm 65
SSR size
223 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide.
Sequence
WMC501
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc501.2
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