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GrainGenes Marker Report: Xwmc503-2D

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Locus	Xwmc503-2D
Type	Microsatellite
Chromosome	2D
Map	CS/SQ1-2D 15.8 Ta-SSR-2004-2D 20.5 USG3209/Jaypee-2D 0
	[ Show Nearby Loci ]
Map Data	Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004 Wheat, USG3209 x Jaypee
Probe	WMC503
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc503 wmc503 wmc503-2D wmc503-3A
Probe for Locus	WMC503
Locus	Xwmc503-2D Xwmc503 wmc503 wmc503-2D wmc503-3A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20b8 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 237 bases located on 2D, 5B Trimmed to insert at both ends. (GT)11 112 to 133, (GT)18 192 to 227.
Type	PCR SSR
PCR primers	Forward gcAATAgTTcccgcAAgAAAAg at 55 length 22 bases, Tm 64 Reverse ATcAAcTAccTccAgATcccgT at 270 length 22 bases, Tm 63
SSR size	237 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide.
Sequence	WMC503
Source Species	Triticum aestivum