GrainGenes Marker Report: Xwmc508-5B
Locus
Xwmc508-5B
Type
Microsatellite
Chromosome
5B
Map
Ta-SSR-2004-5B 140.9
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC508
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc508 wmc508
Probe for Locus
WMC508
Locus
Xwmc508 Xwmc508-5B wmc508
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 20e10 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 181 bases located on 5B Trimmed to insert at 3' end, 5' end not located. Bases 123 to 194 show homology to 8h11 bases 116 to 187 (72 in longest run, maxScore = 63), reverse primer is outside this region of homology, forward primer matches 8h11. (GA)6 83 to 94.
Type
PCR SSR
PCR primers
Forward AgcccTTgAgTTggTcTcATTT at 53 length 22 bases, Tm 63
Reverse gAgcAgAgcTccAcTcAcATTT at 210 length 22 bases, Tm 63
SSR size
179 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product.
Sequence
WMC508
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc508-5B
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