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GrainGenes Marker Report: Xwmc511-4B

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Locus	Xwmc511-4B
Type	Microsatellite
Chromosome	4B
Chromosome Arm	4BS
Map	Ta-SSR-2004-4B 20.3 Triticum-WxM-FHB-QTL-4B 4
	[ Show Nearby Loci ]
Map Data	Wheat, Consensus SSR, 2004 Wheat, WxM, FHB QTL
Probe	WMC511
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114. Somers DJ et al. (2003) Molecular mapping of novel genes controlling Fusarium head blight resistance and deoxynivalenol accumulation in spring wheat Genome 46:555-564.
Possible Orthologs	Xwmc511a wmc511 wmc511-4B
Probe for Locus	WMC511
Locus	Xwmc511a Xwmc511-4B wmc511 wmc511-4B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 20g7 Donor: Byrne, Colorado State Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 125 bases located on 4B, 130 bases located on 4B, 205 bases located on 4B Trimmed to insert at 3' end, 5 ' end not located. (GT)7 148 to 161, (GT)4 172 to 179, also complex CGTG repeats. Related to 23b5, reverse primer has 4 mismatches to 23b5, forward primer has no match.
Type	PCR SSR
PCR primers	Forward cgcAcTcgcATgATTTTccT at 75 length 20 bases, Tm 65 Reverse ATgcccggAAAcgAgAcTgT at 257 length 20 bases, Tm 66
SSR size	202 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC511
Source Species	Triticum aestivum