GrainGenes Marker Report: Xwmc517-7B
Locus
Xwmc517-7B
Type
Microsatellite
Chromosome
7B
Map
Louise/Penawawa-7B 0 P91193D1/P92201D5-7B 247.5 Ta-SSR-2004-7B 92.5 Wheat_Yr79_2018-7BL 77.5
[ Show Nearby Loci ]
Map Data
Durum wheat, Svevo x Ciccio Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, P91193D1 x P92201D5 Wheat_Yr79_2018
Probe
WMC517
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc517 wmc517-7B wmc517 wmc517-4A
Probe for Locus
WMC517
Locus
Xwmc517-7B Xwmc517 wmc517-7B wmc517 wmc517-4A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22h1 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 186 bases located on 7B Trimmed to insert at 5' end and to Ssp1 at 3' end. Bases 219 to 232 show homology to 7g4 bases 201 to 214 (14 in longest run, maxScore = 41). Reverse primer has 5 mismatches to 7g4, forward primer no apparent homology. (CA)16 36 to 67, (CA)6 113 to 124.
Type
PCR SSR
PCR primers
Forward ATccTgAcgTTAcAcgcAcc at 5 length 20 bases, Tm 63
Reverse AccTggAAcAccAcgAcAAA at 173 length 20 bases, Tm 63
SSR size
188 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (180-200 bases)
Sequence
WMC517
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc517-7B
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