GrainGenes Marker Report: Xwmc522-2A
Locus
Xwmc522-2A
Type
Microsatellite
Chromosome
2A
Map
Louise/Penawawa-2A 0 Ta-SSR-2004-2A 45 USG3209/Jaypee-2A 81.3
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa Wheat, USG3209 x Jaypee
Probe
WMC522
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc522 wmc522-2A wmc522 wmc522-3D
Probe for Locus
WMC522
Locus
Xwmc522-2A Xwmc522 wmc522-2A wmc522 wmc522-3D
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22h5 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 2A Trimmed to insert at both ends. (CT)36 74 to 145, (TC)4 163 to 170.
Type
PCR SSR
PCR primers
Forward AAAAATcTcAcgAgTcgggc at 5 length 20 bases, Tm 63
Reverse cccgAgcAggAgcTAcAAAT at 178 length 20 bases, Tm 63
SSR size
193 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (180-200 bases).
Sequence
WMC522
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc522-2A
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