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GrainGenes Marker Report: Xwmc524

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Locus	Xwmc524
Type	Microsatellite
Map	Ta-NxW-5A 242.3 Wheat, UC1110 x PI610750 5A 178.2
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC524
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc524-5A wmc524 wmc524-5A
Probe for Locus	WMC524
Locus	Xwmc524-5A Xwmc524 wmc524 wmc524-5A
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 22f6 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5A Trimmed to insert at both ends. Bases 204 to 215 (complement) show homology to 3e11 bases 124 to 135 (12 in longest run, maxScore = 19). (GT)13 233 to 258, (GT)7 261 to 274, (GT)4 316 to 323.
Type	PCR SSR
PCR primers	Forward TAgTccAccggAcggAAAgTAT at 165 length 22 bases, Tm 63 Reverse gTAccAccgATTgATgcTTgAg at 341 length 22 bases, Tm 63
SSR size	198 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic on acrylamide (160-200 bases).
Sequence	WMC524
Source Species	Triticum aestivum