GrainGenes Marker Report: Xwmc524-5A
Locus
Xwmc524-5A
Type
Microsatellite
Chromosome
5A
Map
Kofa/UC1113-5A 197.7 Ta-SSR-2004-5A 152
[ Show Nearby Loci ]
Map Data
Durum wheat, Kofa x UC1113 Wheat, Consensus SSR, 2004
Probe
WMC524
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc524 wmc524 wmc524-5A
Probe for Locus
WMC524
Locus
Xwmc524-5A Xwmc524 wmc524 wmc524-5A
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22f6 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5A Trimmed to insert at both ends. Bases 204 to 215 (complement) show homology to 3e11 bases 124 to 135 (12 in longest run, maxScore = 19). (GT)13 233 to 258, (GT)7 261 to 274, (GT)4 316 to 323.
Type
PCR SSR
PCR primers
Forward TAgTccAccggAcggAAAgTAT at 165 length 22 bases, Tm 63
Reverse gTAccAccgATTgATgcTTgAg at 341 length 22 bases, Tm 63
SSR size
198 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (160-200 bases).
Sequence
WMC524
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc524-5A
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