GrainGenes Marker Report: Xwmc525-7A
Locus
Xwmc525-7A
Type
Microsatellite
Chromosome
7A
Map
Ta-SSR-2004-7A 112.6
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC525
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc525 wmc525
Probe for Locus
WMC525
Locus
Xwmc525 Xwmc525-7A wmc525
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 22g6 Donor: Bright, Zeneca Trimmed to insert at both ends. Bases 51 to 63 show homology to 25c11 bases 41 to 53 (13 in longest run, maxScore = 22). (GT)13 273 to 298, (GT)20 301 to 340.
Type
PCR SSR
PCR primers
Forward gTTTgAcgTgTTTgcTgcTTAc at 168 length 22 bases, Tm 62
Reverse cTAcggATAATgATTgcTggcT at 352 length 22 bases, Tm 62
SSR size
206 bases expected, Chinese Spring
Amplification Conditions
61C anneal, polymorphic on acrylamide (200-220 bases - stuttery).
Sequence
WMC525
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc525-7A
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