Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc527a

[Submit comment/correction]

Locus	Xwmc527a
Type	Microsatellite
Chromosome	3B
Map	Svevo/Ciccio-3B 106.1 Wheat-Composite2004-3B 50
	[ Show Nearby Loci ]
Breakpoint Interval	C-3BL7-0.63
Map Data	Durum wheat, Svevo x Ciccio Wheat, Composite, 2004
Probe	WMC527
Possible Orthologs	wmc527 [ Show all 9 ]
Probe for Locus	WMC527
Locus	Xwmc527 [ Show all 10 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24B10 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 378 bases located on 3B Trimmed to insert at 5' end and to Ssp1 at 3' end. (CA)15 328 to 357, (GA)20 30 to 69.
Type	PCR SSR
PCR primers	Forward AcccAAgATTggTTgcAgAA at 4 length 20 bases, Tm 63 Reverse gcTAcAgAAAAccggAgccTAT at 366 length 22 bases, Tm 62
SSR size	386 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product (360 bases).
Sequence	WMC527
Source Species	Triticum aestivum