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GrainGenes Marker Report: Xwmc529

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Locus	Xwmc529
Type	Microsatellite
Chromosome	3D
Map	Ta-NxW-3B 100.2 Wheat-Composite2004-3D 53
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Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC529
Possible Orthologs	Xwmc529-3D
Probe for Locus	WMC529
Locus	Xwmc529 Xwmc529-3D
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 24F10 Donor: Bright, Zeneca Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 189 bases located on 3D Trimmed to Ssp1 at 5' end and to insert at 3' end. (CA)13 95 to 120.
Type	PCR SSR
PCR primers	Forward ATTgcATgcAAATTAgTAgTAg at 7 length 22 bases, Tm 53 Reverse gTgTTgAcAAATTTTgAgTTAg at 171 length 22 bases, Tm 54
SSR size	180 bases expected, Chinese Spring
Sequence	WMC529
Source Species	Triticum aestivum