GrainGenes Marker Report: Xwmc537-5B
Locus
Xwmc537-5B
Type
Microsatellite
Chromosome
5B
Map
Conan/Reeder-5B 41.4873 Louise/Penawawa-5B 68 Ta-SSR-2004-5B 84
[ Show Nearby Loci ]
Map Data
Wheat, Conan x Reeder Wheat, Consensus SSR, 2004 Wheat, Louise x Penawawa
Probe
WMC537
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc537 wmc537 wmc537-5B
Probe for Locus
WMC537
Locus
Xwmc537-5B Xwmc537 wmc537 wmc537-5B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 26d3 Donor: Madre, Secobra Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 180 bases located on 5B, 7B Trimmed to Ssp1 at 5' end and to insert at 3' end. Bases 66 to 121 (complement) show homology to 3e3 bases 837 to 892 (56 in longest run, maxScore = 47). Primers have apparently no homology to 3e3. Bases 8 to 228 show homology to 26e3 bases 7 to 227 (221 in longest run, maxScore = 133). (CA)26 157 to 208, (CT)10 125 to 144.
Type
PCR SSR
PCR primers
Forward TcTTcTgTAcATTgAAcAAcgA at 56 length 22 bases, Tm 58
Reverse ATgcAgAAccgTgATAggAT at 208 length 20 bases, Tm 59
SSR size
170 bases expected, Chinese Spring
Amplification Conditions
51C anneal, polymorphic on acrylamide (98-106 bases and 176-184 bases).
Sequence
WMC537
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc537-5B
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
| ||||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
| |||||||
|
|