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GrainGenes Marker Report: Xwmc539-6B

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Locus	Xwmc539-6B
Type	Microsatellite
Chromosome	6B
Map	Ta-SSR-2004-6B 47.4 Wheat_Yr78_2017-6B 54
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Map Data	Wheat, Consensus SSR, 2004 Wheat_Yr78_2017
Probe	WMC539
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc539 wmc539 wmc539-6B
Probe for Locus	WMC539
Locus	Xwmc539 Xwmc539-6B wmc539 wmc539-6B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 26a6 Donor: Madre, Secobra Trimmed to insert at both ends. (GT)64 75 to 202.
Type	PCR SSR
PCR primers	Forward gcAAgTAggAccTTAcAgTTcT at 27 length 22 bases, Tm 56 Reverse gTTATAAccTTTgTcccTTcAc at 204 length 22 bases, Tm 56
SSR size	199 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product (200 bases).
Sequence	WMC539
Source Species	Triticum aestivum