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GrainGenes Marker Report: Xwmc54

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Locus	Xwmc54
Type	Microsatellite
Chromosome	3B
Map	CIMMYT-integrated-3B 69.74 Ta-NxW-3B 75.6 Wheat-Composite2004-3B 23
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Map Data	Wheat, CIMMYT Integrated Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai
Probe	WMC54
Possible Orthologs	wmc54
Probe for Locus	WMC54
Locus	Xwmc54 wmc54
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1c7 Donor: Waugh, SCRI (GT)8 176 to 191, (GT)8 194 to 209. Bands tend to be faint.
Type	PCR SSR
PCR primers	Forward TATTgTgcAATcgcAgcATcTc at 141 length 22 bases, Tm 65 Reverse TgcgAcATTggcAAccAcTTcT at 261 length 22 bases, Tm 69
SSR size	142 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC54
Source Species	Triticum aestivum