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GrainGenes Marker Report: Xwmc581

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Locus	Xwmc581
Type	Microsatellite
Map	CIMMYT-integrated-7B 119.1 Svevo/Ciccio-7B 150
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Breakpoint Interval	7BL10-0.78-1.00
Map Data	Durum wheat, Svevo x Ciccio Wheat, CIMMYT Integrated
Probe	WMC581
Possible Orthologs	Xwmc581-7B wmc581 wmc581-7B
Probe for Locus	WMC581
Locus	Xwmc581-7B Xwmc581 wmc581 wmc581-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15h3.2 Donor: Aventis Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 104 to 161 (complement) show homology to 6g11 bases 194 to 251 (58 in longest run, maxScore = 55). (GT)23 36 to 81, (GT)8 83 to 98. Reverse primer matches 6g11 sequence. Foward primer is unique.
Type	PCR SSR
PCR primers	Forward cATgTTgccATcAAAcTcgc at 10 length 20 bases, Tm 64 Reverse gctaTTgAcATgcAAcTATggAcct at 108 length 25 bases, Tm 64
SSR size	123 bases expected, Chinese Spring
Sequence	WMC581
Source Species	Triticum aestivum