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GrainGenes Marker Report: Xwmc581-7B

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Locus	Xwmc581-7B
Type	Microsatellite
Chromosome	7B
Map	CS/SQ1-7B 188.5 Kofa/UC1113-7B 183.4 Ta-SSR-2004-7B 137.9
	[ Show Nearby Loci ]
QTL with Locus Marker	QYld.saq-7B
Map Data	Durum wheat, Kofa x UC1113 Durum wheat, Svevo x Ciccio Wheat, Chinese Spring x SQ1 Wheat, Consensus SSR, 2004
Probe	WMC581
Reference	Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs	Xwmc581 wmc581 wmc581-7B
Probe for Locus	WMC581
Locus	Xwmc581-7B Xwmc581 wmc581 wmc581-7B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 15h3.2 Donor: Aventis Multiple inserts in this clone, this is the second insert. Trimmed to insert at both ends. Bases 104 to 161 (complement) show homology to 6g11 bases 194 to 251 (58 in longest run, maxScore = 55). (GT)23 36 to 81, (GT)8 83 to 98. Reverse primer matches 6g11 sequence. Foward primer is unique.
Type	PCR SSR
PCR primers	Forward cATgTTgccATcAAAcTcgc at 10 length 20 bases, Tm 64 Reverse gctaTTgAcATgcAAcTATggAcct at 108 length 25 bases, Tm 64
SSR size	123 bases expected, Chinese Spring
Sequence	WMC581
Source Species	Triticum aestivum