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GrainGenes Marker Report: Xwmc68

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Locus	Xwmc68
Type	Microsatellite
Map	Ta-NxW-4B 127.4
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai
Probe	WMC68
Probe for Locus	WMC68
Locus	Xwmc68
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1a12 Donor: Waugh, SCRI (GT)19 33 to 70. If polymorphic then dominant.
Type	PCR SSR
PCR primers	Forward TAcAccTcgcgTgTgTAgccAA at 3 length 22 bases, Tm 68 Reverse gcTcgAATTcTggcTcggcAAc at 184 length 22 bases, Tm 71
SSR size	203 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC68
Source Species	Triticum aestivum