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GrainGenes Marker Report: Xwmc73b

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Locus	Xwmc73b
Type	Microsatellite
Chromosome	5B
Map	Wheat-Composite2004-5B 29
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC73
Possible Orthologs	Xwmc73 Xwmc73-5B wmc73 wmc73-5B
Probe for Locus	WMC73
Locus	Xwmc73-5B Xwmc73 Xwmc73b wmc73 wmc73-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1h12 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 173 bases located on 5B, 192 bases located on 5B, 579 bases located on 5B (CA)9 53 to 70. (3 out of 4 loci polymorphic?)
Type	PCR SSR
PCR primers	Forward TTgTgcAccgcAcTTAcgTcTc at 7 length 22 bases, Tm 67.0 Reverse AcAcccggTcTccgATccTTAg at 174 length 22 bases, Tm 67.0
SSR size	189 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC73
Source Species	Triticum aestivum