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GrainGenes Marker Report: Xwmc75

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Locus	Xwmc75
Type	Microsatellite
Chromosome	5B
Map	Ta-NxW-5B 158.4 Wheat, UC1110 x PI610750 5B 72.9 Wheat-Composite2004-5B 89
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004 Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC75
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc75-5B wmc75 wmc75-5B
Probe for Locus	WMC75
Locus	Xwmc75 Xwmc75-5B wmc75 wmc75-5B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6c7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5B AGR99. Fragment trimmed to insert(GT)13 117 to 142. Matches 3a6.
Type	PCR SSR
PCR primers	Forward gTccgccgcAcAcATcTTAcTA at 51 length 22 bases, Tm 66 Reverse gTTTgATccTgcgAcTcccTTg at 235 length 22 bases, Tm 67
SSR size	206 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC75
Source Species	Triticum aestivum