GrainGenes Marker Report: Xwmc75-5B
Locus
Xwmc75-5B
Type
Microsatellite
Chromosome
5B
Map
Ta-SSR-2004-5B 113.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC75
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc75 wmc75 wmc75-5B
Probe for Locus
WMC75
Locus
Xwmc75 Xwmc75-5B wmc75 wmc75-5B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6c7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 207 bases located on 5B AGR99. Fragment trimmed to insert(GT)13 117 to 142. Matches 3a6.
Type
PCR SSR
PCR primers
Forward gTccgccgcAcAcATcTTAcTA at 51 length 22 bases, Tm 66
Reverse gTTTgATccTgcgAcTcccTTg at 235 length 22 bases, Tm 67
SSR size
206 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product;
Sequence
WMC75
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc75-5B
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