GrainGenes Marker Report: Xwmc78-3B
Locus
Xwmc78-3B
Type
Microsatellite
Chromosome
3B
Map
Ta-SSR-2004-3B 50.7
[ Show Nearby Loci ]
Map Data
Wheat, Consensus SSR, 2004
Probe
WMC78
Reference
Somers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc78 wmc78
Probe for Locus
WMC78
Locus
Xwmc78 Xwmc78-3B wmc78
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6f7 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 265 bases located on 3B AGR102. Trimmed to insert. (CA)18 158 to 193. (2 loci - 1 polymorph) Bases 158 to 282 show homology to 2a3 bases 183 to 307 (125 in longest run, maxScore = 79). True duplicate with 2a3, 6f7 has priority.
Type
PCR SSR
PCR primers
Forward AgTAAATccTcccTTcggcTTc at 42 length 22 bases, Tm 64
Reverse AgcTTcTTTgcTAgTccgTTgc at 261 length 22 bases, Tm 64
SSR size
241 bases expected, Chinese Spring
Amplification Conditions
tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC78
Source Species
Triticum aestivum
GrainGenes Marker Report: Xwmc78-3B
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