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GrainGenes Marker Report: Xwmc83

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Locus	Xwmc83
Type	Microsatellite
Map	Ta-NxW-7A 133.4 Wheat, UC1110 x PI610750 7A 89.1 Wheat, WH542 x Synthetic 7A 154.1
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750 Wheat, WH542 x Synthetic
Probe	WMC83
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc83-2B [ Show all 8 ]
Probe for Locus	WMC83
Locus	Xwmc83-7A [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6f8 Donor: Devaux, Florimond Desprez Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 166 bases located on 7A AGR110. Trimmed to insert.(GT)28 32 to 87. (4 polymorphic loci?).
Type	PCR SSR
PCR primers	Forward TggAggAAAcAcAATggATgcc at 7 length 22 bases, Tm 68 Reverse gAgTATcgccgAcgAAAgggAA at 145 length 22 bases, Tm 68
SSR size	160 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC83
Source Species	Triticum aestivum