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GrainGenes Marker Report: Xwmc85a

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Locus	Xwmc85a
Type	Microsatellite
Chromosome	1B
Map	Wheat-Composite2004-1B 22
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC85
Possible Orthologs	wmc85 [ Show all 13 ]
Probe for Locus	WMC85
Locus	Xwmc85-1B [ Show all 14 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g9 Donor: Devaux, Florimond Desprez AGR119. Trimmed to insert at 3' end(CA)8 153 to 168, (CA)18 184 to 219. Products a little weak at 61C, try lower anneal?
Type	PCR SSR
PCR primers	Forward ggAgTAAgAgAAAcATgccgAA at 27 length 22 bases, Tm 62 Reverse gTgcATgcATgAgAATAggAAc at 233 length 22 bases, Tm 61
SSR size	228 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC85
Source Species	Triticum aestivum