Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Marker Report: Xwmc95b

[Submit comment/correction]

Locus	Xwmc95b
Type	Microsatellite
Chromosome	1A
Map	Wheat-Composite2004-1A 35
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC95
Possible Orthologs	Xwmc95 [ Show all 8 ]
Probe for Locus	WMC95
Locus	Xwmc95-1A [ Show all 9 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7f1 Donor: Somers, Agriculture Canada Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 178-211 bases located on 1A, 205 bases located on 6B Trimmed to insert at both ends. (GT)18 179 to 214. A struggle to fit primers. (3 polymorphic loci?) Bases 83 to 173 show homology to 19d5 bases 1 to 91 (91 in longest run, maxScore = 75).
Type	PCR SSR
PCR primers	Forward gTTTTTgTgATcccgggTTT at 53 length 20 bases, Tm 63 Reverse cATgcgTcAgTTcAAgTTTT at 229 length 20 bases, Tm 58
SSR size	196 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC95
Source Species	Triticum aestivum