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GrainGenes Marker Report: Xwmc96

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Locus	Xwmc96
Type	Microsatellite
Map	Ta-NxW-5A 70.5 Wheat, UC1110 x PI610750 5A 41.5
	[ Show Nearby Loci ]
Map Data	Wheat, Nanda2419 x Wangshuibai Wheat, UC1110 x PI610750
Probe	WMC96
Reference	Lowe I et al. (2011) Mapping and validation of QTL which confer partial resistance to broadly virulent post-2000 North American races of stripe rust in hexaploid wheat Theoretical and Applied Genetics 123:143-157.
Possible Orthologs	Xwmc96-3A [ Show all 15 ]
Probe for Locus	WMC96
Locus	Xwmc96-4A [ Show all 16 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d2 Donor: Somers, Agriculture Canada Trimmed to insert at both ends. (CA)29 177 to 234. (1 locus polymophic out of 3)
Type	PCR SSR
PCR primers	Forward TAgcAgccATgcTTAgcATcAA at 28 length 22 bases, Tm 65 Reverse gTTTcAgTcTTTcAcgAAcAcg at 286 length 22 bases, Tm 62
SSR size	280 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC96
Source Species	Triticum aestivum