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GrainGenes Marker Report: Xwmc96-3A

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Locus
Xwmc96-3A
Type
Microsatellite
Chromosome
3A
Map
T.b.xT.m.-3A140.6
Ta-SSR-2004-3A71.5
[ Show Nearby Loci ]
Map Data
T. boeoticum x monococcum
Wheat, Consensus SSR, 2004
Probe
WMC96
Reference
ReferenceSomers DJ et al. (2004) A high-density wheat microsatellite consensus map for bread wheat (Triticum aestivum L.) Theoretical and Applied Genetics 109:1105-1114.
Possible Orthologs
Xwmc96
[ Show all 15 ]



Probe for Locus
WMC96
Locus
Xwmc96-4A
[ Show all 16 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 7d2
Donor: Somers, Agriculture Canada
Trimmed to insert at both ends. (CA)29 177 to 234. (1 locus polymophic out of 3)
Type
PCR
SSR
PCR primers
Forward TAgcAgccATgcTTAgcATcAA at 28 length 22 bases, Tm 65
Reverse gTTTcAgTcTTTcAcgAAcAcg at 286 length 22 bases, Tm 62
SSR size
280 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC96
Source Species
Triticum aestivum