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GrainGenes Marker Report: Xwmc96e

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Locus	Xwmc96e
Type	Microsatellite
Chromosome	5A
Map	Wheat-Composite2004-5A 76
	[ Show Nearby Loci ]
Map Data	Wheat, Composite, 2004
Probe	WMC96
Possible Orthologs	Xwmc96 [ Show all 15 ]
Probe for Locus	WMC96
Locus	Xwmc96-4A [ Show all 16 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 7d2 Donor: Somers, Agriculture Canada Trimmed to insert at both ends. (CA)29 177 to 234. (1 locus polymophic out of 3)
Type	PCR SSR
PCR primers	Forward TAgcAgccATgcTTAgcATcAA at 28 length 22 bases, Tm 65 Reverse gTTTcAgTcTTTcAcgAAcAcg at 286 length 22 bases, Tm 62
SSR size	280 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC96
Source Species	Triticum aestivum