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GrainGenes Marker Report: cMWG679

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Locus	cMWG679
Type	CAPS RFLP
Synonym	Synonym POPA2_0232
Chromosome	6H
Chromosome Arm	6HS
Map	Barley-Consensus-6H -3.3 [ Show all 8 ]
	[ Show Nearby Loci ]
Map Data	Barley consensus [ Show all 8 ]
Species	Hordeum vulgare
Probe	cMWG679
Reference	Abu Qamar M et al. (2008) A region of barley chromosome 6H harbors multiple major genes associated with net type net blotch resistance. Theoretical and Applied Genetics 117:1261-1270.
Data Source	Kleinhofs, Andris
Remark	This marker is a Binlocus in the Hordeum-Bins maps, i.e. a marker mapped in other crosses and located to the Steptoe x Morex bin Bin on the 'Barley, Integrated, Marcel 2009' Map Set : 'chromosome_kleinhofs & graner BIN nb.subBIN nb' Mapping population(s) used for locus placement on the 'Barley, Integrated, Marcel 2009' Map Set = Igri x Franka Loci in 'Barley, Consensus 2006, Marcel' mapped in population(s) : Igri x Franka
Possible Orthologs	cMWG679-6H Xcmwg679-6A.1 Xcmwg679-6A.2
Probe	cMWG679
Locus	cMWG679 Xcmwg679-6A.1 Xcmwg679-6A.2 cMWG679-6H
Synonym	Synonym HAR35_809 HAR35_810 HAR35_811
Reference	Abu Qamar M et al. (2008) A region of barley chromosome 6H harbors multiple major genes associated with net type net blotch resistance. Theoretical and Applied Genetics 117:1261-1270. Kunzel G et al. (2000) Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints Genetics 154:397-412. Graner A et al. (1991) Construction of an RFLP map of barley Theoretical and Applied Genetics 83:250-256.
Remarks	Restriction enzyme informative in CAP analysis is RsaI
Type	cDNA STS
PCR primers	TCAAGGCTAACCCCATGTTC CCCATGAAGATGAGTGCAT
STS primers	5' GAGCAGCCCGTGACATGGAA 5' CTCCACCAGTGCAACCTCGT
STS size	196
Amplification Conditions	STS annealing temperature 60-62 oC
Sequence	AJ234417 AJ234416 CMWG679-5 CMWG679-3
DNA Library	cMWG
Source Species	Hordeum vulgare
Source Tissue	Leaf