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GrainGenes Marker Report: gwm112b

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Locus	gwm112b
Type	Microsatellite
Map	Physical-3B 90.95
	[ Show Nearby Loci ]
Map Data	3B Physical
Probe	WMS112
Possible Orthologs	Xgwm112 [ Show all 8 ]
Probe for Locus	WMS112
Locus	Xgwm112-7B [ Show all 9 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)8GT(CT)20 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' CTAAACACGACAGCGGTGG 3' 5' GATATGTGAGCAGCGGTCAG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS112
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring