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GrainGenes Marker Report: gwm148

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Locus	gwm148
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 2B 65 Physical-3B 7.82 Wheat, Yr genes and QTL 2B 30.4 Wheat_Consensus_2014_Ug99_Rust_2B 89.02 Wheat_Yr_2021-2B 100.04
	[ Show Nearby Loci ]
QTL with Locus Marker	QHi.OGxM1-2B.2 QSHi.OGxM1-2B.2
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat, Yr Update, 2021 Wheat_Consensus_2014_Ug99_Rust
Probe	WMS148
Possible Orthologs	Xgwm148 Xgwm148-2B gwm148-2B
Probe for Locus	WMS148
Locus	Xgwm148-2B Xgwm148 Ne2 gwm148 gwm148-2B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)22 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTGAGGCAGCAAGAGAGAAA 3' 5' CAAAGCTTGACTCAGACCAAA 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS148
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring