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GrainGenes Marker Report: gwm181

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Locus	gwm181
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 211.9 Physical-3B 178.6 Wheat, Yr genes and QTL 3B 100 Wheat_YrH922_2019_exp 22.8 Wheat_Yr_2021-3B 213.42
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat, Yr Update, 2021 Wheat_YrH922_2019
Probe	WMS181
Possible Orthologs	Xgwm181 Xgwm181-3B gwm181-3B
Probe for Locus	WMS181
Locus	Xgwm181 Xgwm181-3B gwm181 gwm181-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GA)28 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TCATTGGTAATGAGGAGAGA 3' 5' GAACCATTCATGTGCATGTC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS181
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring