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GrainGenes Marker Report: gwm264

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Locus	gwm264
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 66.3 Physical-3B 44.8 Wheat, Yr genes and QTL 1B 34.9 Wheat, Yr genes and QTL 3B 36.5 Wheat_Consensus_2014_Ug99_Rust_1B 21.38
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat_Consensus_2014_Ug99_Rust
Probe	WMS264
Possible Orthologs	Xgwm264 [ Show all 14 ]
Probe for Locus	WMS264
Locus	Xgwm264-1B [ Show all 15 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)9A(CA)24 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GAGAAACATGCCGAACAACA 3' 5' GCATGCATGAGAATAGGAACTG 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS264
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring