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GrainGenes Marker Report: gwm274

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Locus	gwm274
Type	microsatellite
Map	Physical-3B 60.04 [ Show all 6 ]
	[ Show Nearby Loci ]
Map Data	3B Physical Wheat AB, Carotenoid QTL, 2017 Wheat, Yr genes and QTL
Probe	WMS274
Possible Orthologs	Xgwm274 [ Show all 14 ]
Probe for Locus	WMS274
Locus	Xgwm274-1B [ Show all 15 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (GT)27 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' AACTTGCAAAACTGTTCTGA 3' 5' TATTTGAAGCGGTTTGATTT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 50 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS274
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring