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GrainGenes Marker Report: gwm4

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Locus	gwm4
Type	microsatellite
Map	Durum Wheat, NSGC Lr 7A 130.5 [ Show all 7 ]
	[ Show Nearby Loci ]
Map Data	3B Physical Durum Wheat, NSGC Lr Wheat AB, Carotenoid QTL, 2017 Wheat, Yr genes and QTL
Probe	WMS4
Possible Orthologs	Xgwm4-3A [ Show all 10 ]
Probe for Locus	WMS4
Locus	Xgwm4-4A [ Show all 11 ]
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)13(TA)26 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GCTGATGCATATAATGCTGT 3' 5' CACTGTCTGTATCACTCTGCT 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS4
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring