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GrainGenes Marker Report: gwm547

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Locus	gwm547
Type	Microsatellite
Map	Durum Wheat, NSGC Lr 3B 209.3 Physical-3B 178.6 Wheat, Yr genes and QTL 3B 98.1 Wheat_Consensus_2014_Ug99_Rust_3B 182.16 Wheat_YrH922_2019_exp 21.5
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Map Data	3B Physical Durum Wheat, NSGC Lr Wheat, Yr genes and QTL Wheat_Consensus_2014_Ug99_Rust Wheat_YrH922_2019
Probe	WMS547
Possible Orthologs	Xgwm547 Xgwm547-2B Xgwm547-3B gwm547-3B
Probe for Locus	WMS547
Locus	Xgwm547-3B Xgwm547-2B Xgwm547 gwm547 gwm547-3B
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CA)12 PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' GTTGTCCCTATGAGAAGGAACG 3' 5' TTCTGCTGCTGTTTTCATTTAC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 60 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS547
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring