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GrainGenes Marker Report: gwm72

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Locus	gwm72
Type	Microsatellite
Map	Physical-3B 58.4 Wheat, Yr genes and QTL 3B 37.3 Wheat_Yr_2021-3B 79.58
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Map Data	3B Physical Wheat, Yr genes and QTL Wheat, Yr Update, 2021
Probe	WMS72
Possible Orthologs	Xgwm72 Xgwm72-3B
Probe for Locus	WMS72
Locus	Xgwm72-3B Xgwm72 gwm72
Reference	Roder MS et al. (1998) A microsatellite map of wheat Genetics 149:2007-2023.
General Remarks	Insert contains repeat (CT)48imp PRIMER SEQUENCE IS AVAILABLE FOR PUBLIC RESEARCH ONLY. REQUESTS FOR COMMERCIAL USE SHOULD BE DIRECTED TO M. ROEDER.
Type	PCR SSR
PCR primers	5' TGGTCCCTCTCCCTTTCTCT 3' 5' ACAGAATTGAAGATTGTCGGTC 3'
Amplification Conditions	3 min at 94 deg C; 45 cycles with 1 min at 94 deg C, 1 min at 55 deg C, and 2 min at 72 deg C; and a final extension step of 10 min at 72 deg C
Polymorphism	WMS72
Source Species	Triticum aestivum
Source Germplasm	Chinese Spring