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GrainGenes Marker Report: wmc7

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Locus	wmc7
Type	Microsatellite
Map	Physical-3B 58.92
	[ Show Nearby Loci ]
Map Data	3B Physical
Probe	WMC7
Possible Orthologs	Xwmc007
Probe	WMC7
Locus	wmc7 Xwmc007
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1E3 Donor: Nickerson AGR21. Trimmed to Rsa1 fragment. Multple inserts (2). Complex (C)(CA)(GA) repeat in second fragment. Last insert highly homologous to 1B5. Bases 1 to 32 show homology to 19a6 bases 133 to 164 (32 in longest run, maxScore = 27).
Type	PCR SSR
PCR primers	Forward gcTTggAcTAgcTAgAgTATcAT at 356 length 23 bases, Tm 56 Reverse AcAATcTTcAATTcTgTcgcAcg at 536 length 23 bases, Tm 65
SSR size	203 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC7
Source Species	Triticum aestivum