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GrainGenes Probe Report: BS00003705_51

[What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	BS00003705_51
Locus	BS00003705 IWB5871
Reference	Wang S et al. (2014) Characterization of polyploid wheat genomic diversity using a high-density 90 000 single nucleotide polymorphism array. Plant Biotechnology Journal 12:787-796. Allen AM et al. (2011) Transcipt-specific, single-nucleotide polymorphism discovery and linkage analysis in hexaploid bread wheat (Triticum aestivum L.) Plant Biotechnology Journal 9:1086-1099.
Type	SNP KASP
PCR primers	FAM 5' GTACGGTACTGATGGATGATGTACA 3' Common 5' GCATCGTTCCGAATTATGGCTCTTAATTT 3' VIC 5' GTACGGTACTGATGGATGATGTACT 3' Common 5' GCATCGTTCCGAATTATGGCTCTTAATTT 3'
Specificity	FAM primer specific to A allele, and VIC primer specific to T allele.
Sequence	BS00003705_51