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GrainGenes Probe Report: Bmac399

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Bmac399  [ Marker Report ]
Locus	Bmac0399 Bmac399-1H Bmac399
Synonym	Other name Bmac0399
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AC)21 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.72
Type	PCR SSR
PCR primers	5' CGATGCTTTACTATGAGAGGT 3' 5' GGGTCTGAAGCCTGAAC 3'
SSR size	145
Amplification Conditions	1 cycle of 3 mins @ 94C, 1 min @ 66C, 1 min @ 72C, 5 cycles of 30 secs @ 94C, 30 secs @ 65C (decreasing 1C per cycle), 30 secs @ 72C, 24 cycles of 30 secs @ 94C, 30 secs @ 60C, 30 secs @ 72C, 1 cycle of 5 mins @ 72C.
Source Species	Hordeum vulgare