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GrainGenes Probe Report: Bmag15

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Bmag15  [ Marker Report ]
Locus	Bmag0015
Synonym	Other name Bmag0015
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (AG)8 Quality = 3: Strong major polymorphic product, with additional strong bands. Multi-locus or weak but scoreable products
Type	PCR SSR
PCR primers	5' TTGAGGGCTGAACACTTCG 3' 5' GCCCACTGTCAAGGACAATT 3'
SSR size	183
Amplification Conditions	'Touchdown' PCR: 18 cycles of denaturing 1 min @94C and extension 1 min @72C, with annealing for 30s with temp decreased 1C every second cycel from 69C to 60C. Continue 20 cycles for 1 min @94C, 1 min @55C, 1 min @72C. End with 5 min @72C.
Source Species	Hordeum vulgare