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GrainGenes Probe Report: Bmg1

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	Bmg1  [ Marker Report ]
Locus	Bmg0001 BMG001-6H
Reference	Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (G)10 Quality = 1: Single product, only weak stutter bands Diversity Index (DI) = 0.77
Type	PCR SSR
PCR primers	5' GTGTGGTGTTAGCAAGGGGT 3' 5' TCACAGGGCACACACACAC 3'
SSR size	177
Amplification Conditions	'Touchdown' PCR: 18 cycles of denaturing 1 min @94C and extension 1 min @72C, with annealing for 30s with temp decreased 1C every second cycel from 69C to 60C. Continue 20 cycles for 1 min @94C, 1 min @55C, 1 min @72C. End with 5 min @72C.
Source Species	Hordeum vulgare