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GrainGenes Probe Report: HVHVA1

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	HVHVA1  [ Marker Report ]
Locus	HVHVA1
Reference	Varshney RK et al. (2007) A high density barley microsatellite consensus map with 775 SSR loci. Theoretical and Applied Genetics 114:1091. Macaulay M et al. (2001) A representative, highly informative 'genotyping set' of barley SSRs Theoretical and Applied Genetics 102:801-809. Ramsay L et al. (2000) A simple sequence repeat-based linkage map of barley Genetics 156:1997-2005.
General Remarks	Insert contains repeat (ACC)5 Quality = 2: Single product, relatively strong stutter bands Diversity Index (DI) = 0.34
Type	PCR SSR
PCR primers	5' CATGGGAGGGGACAACAC 3' 5' CGACCAAACACGACTAAAGGA 3' CATGGGAGGGGACAACAC CGACCAAACACGACTAAAGGA
SSR size	136
Amplification Conditions	1 cycle of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 30 cycles of 1 min @ 94C, 1 min @ 55C, 1 min @ 72C, 1 cycle of 5 mins @ 72C.
Sequence	X7805
Source Species	Hordeum vulgare