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GrainGenes Probe Report: WMC15

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC15  [ Marker Report ]
Locus	Xwmc15-4A wmc15
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1H5 Donor: Nickerson AGR40. (CT) (CA) compound microsatellite. expected fragment=295. (2 loci?). Bases 223 to 245 show homology to 21e1 bases 121 to 143 (complement) (23 in longest run, maxScore = 25).
Type	PCR SSR
PCR primers	Forward AgTccgATTcggAcTccTcAAg at 164 length 22 bases, Tm 66 Reverse ggAcTAAccgAgggTAgTTcAg at 437 length 22 bases, Tm 61
SSR size	295 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC15
Source Species	Triticum aestivum