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GrainGenes Probe Report: WMC67

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC67
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1d11 Donor: Waugh, SCRI (CA)8 72 to 87, small microsatellite. From microsatellite there is strong homology to 6C6 , also bases 1-70 ie. 5' side of microsatellite match the complement of part of 9e1and 9d1.
Type	PCR SSR
PCR primers	Forward TAggTcgATcTgTTgcAcTcTc at 26 length 22 bases, Tm 61 Reverse TcAcTTTAgAAggTgcAAcggg at 99 length 22 bases, Tm 65
Amplification Conditions	61C anneal, product;
Sequence	WMC67
Source Species	Triticum aestivum