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GrainGenes Probe Report: WMC68

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC68  [ Marker Report ]
Locus	Xwmc68
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1a12 Donor: Waugh, SCRI (GT)19 33 to 70. If polymorphic then dominant.
Type	PCR SSR
PCR primers	Forward TAcAccTcgcgTgTgTAgccAA at 3 length 22 bases, Tm 68 Reverse gcTcgAATTcTggcTcggcAAc at 184 length 22 bases, Tm 71
SSR size	203 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC68
Source Species	Triticum aestivum