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GrainGenes Probe Report: WMC70

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC70  [ Marker Report ]
Locus	Xwmc70 Xwmc70-7B wmc70
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 1d12 Donor: Waugh, SCRI Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 220 bases located on 7B (CA)22 96 to 139, (CA)24 141 to 188. (1 polymorphic locus). Close relative (duplicate?) of 1d2, 1G4 - do they detect at least 1 common locus?
Type	PCR SSR
PCR primers	Forward ggggAgcAcccTcTATTgTcTA at 22 length 22 bases, Tm 63.0 Reverse TAATgcTcccAggAgAgAgTcg at 213 length 22 bases, Tm 65.0
SSR size	213 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC70
Source Species	Triticum aestivum