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GrainGenes Probe Report: WMC84

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC84  [ Marker Report ]
Locus	Xwmc84 Xwmc084 Xwmc087
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 6g8 Donor: Devaux, Florimond Desprez AGR111. Trimmed to insert at 3'. (GT)27 296 to 349. (2-3 loci, one polymorph)
Type	PCR SSR
PCR primers	Forward TgAcgAcTTcgTAgTAgTgcTT at 201 length 22 bases, Tm 59 Reverse gAATgcTcTcATcATgcAcAAc at 511 length 22 bases, Tm 62
SSR size	332 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC84
Source Species	Triticum aestivum