GrainGenes Probe Report: WMC87
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC87
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 6a11 Donor: Devaux, Florimond Desprez AGR129. Trimmed to insert. (GA)16 195 to 226, (GA)9 347 to 364, (GT)9 177 to 194, (GT)15 317 to 346, (GT)15 400 to 429, (GT)8 433 to 448. Duplicate of 2e11 11e5. Expected band is smeary - smaller polymorphic bands. Weak match to 13H4. Bases 4 to 78 show homology to 17h10 bases 298 to 372 (complement) (75 in longest run, maxScore = 104). Bases 232 to 274 show homology to 21g8 bases 32 to 74 (43 in longest run, maxScore = 38). Bases 350 to 383 show homology to 25e6 bases 35 to 68 (complement) (34 in longest run, maxScore = 28). Bases 86 to 124 show homology to 14g9.1 bases 82 to 120 (39 in longest run, maxScore = 106). Close enough to classify as duplicate of 14g9.1, 6a11 has priority.
Type
PCR SSR
PCR primers
Forward TggATgAAgggTggTAggAAcA at 95 length 22 bases, Tm 65
Reverse AgAgATTAcATccgccAccgAg at 472 length 22 bases, Tm 66
SSR size
399 bases expected, Chinese Spring
Amplification Conditions
61C anneal, smear; 61C anneal, polymorphic on acrylamide;
Sequence
WMC87
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC87
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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